Research interests

Biochemistry and ecology of spider venom ( Cupiennius salei ):

• Identification and characterization of neurotoxins in the venom
• Identification and characterization of antimicrobial peptides in the venom and hemolymph
• Synergistic interactions of spider venom components

 Education

Publications  

Wullschleger B, Nentwig W, Kuhn-Nentwig L,(2005) Spider venom: enhancement of venom efficacy mediated by different synergistic strategies in Cupiennius salei. J Exp Biol 208: 2115-2121

Wullschleger B, Kuhn-Nentwig L, Tromp J, Kämpfer U, Schaller J, Nentwig W (2004) CSTX-13, a highly synergistically acting two-chain neurotoxic enhancer in the venom of the spider Cupiennius salei (Ctenidae). Proc Natl Acad Sci USA101: 11251-11256

Kuhn-Nentwig L , Schaller, J & Nentwig, W (2004) Biochemistry, toxicology and ecology of the venom of the spider Cupiennius salei (Ctenidae). Toxicon 43: 543-553

Kuhn-Nentwig L (2003) Antimicrobial and cytolytic peptides of venomous arthropods. Cell Mol Life Sci 60: 2651-2668

Kuhn-Nentwig L , Dathe M, Walz A, Schaller J & Nentwig W (2002) Cupiennin 1d*: the cytolytic activity depends on the hydrophobic N-terminus and is modulated by the polar C-terminus. FEBS Lett 527: 193-198

Kuhn-Nentwig L , Müller J, Schaller J, Walz A, Dathe M & Nentwig W (2002) Cupiennin 1, a new family of highly basic antimicrobial peptides in the venom of the spider Cupiennius salei (Ctenidae). J Biol Chem 277: 11208-11216

Wigger E, Kuhn-Nentwig L & Nentwig W (2002) The venom optimisation hypothesis: a spider injects large venom quantities only into difficult prey types. Toxicon 40: 749-752

Schürch S, Schaller J, Kämpfer U, Kuhn-Nentwig L & Nentwig W (2001) Neurotoxic peptides in the multicomponent venom of the spider Cupiennius salei - Part II. Elucidation of the disulphide-bridge pattern of the neurotoxic peptide CSTX-9 by tandem mass spectrometry. Chimia 55: 1063-1066

Schaller J, Kuhn-Nentwig L, Schürch S, Kämpfer U, Müller J & Nentwig W (2001) Neurotoxic peptides in the multicomponent venom of the spider Cupiennius salei - Part I. Primary structure of neurotoxic peptides in relation to their biological function. Chimia 55: 1058-1062

Kuhn-Nentwig L , Schaller J, Walz A, & Nentwig W (2001) European patent Nr. 018 107 94.6

Schaller J, Kämpfer U, Schürch S, Kuhn-Nentwig L, Haeberli S & Nentwig W (2001) CSTX-9, a toxic peptide from the spider Cupiennius salei: amino acid sequence, disulphide bridge pattern and comparison with other spider toxins containing the cystine knot structure. Cell Mol Life Sci 58: 1538-1545

Mafra RA, Kuhn-Nentwig L, Beirão PSL, Araújo DAM & Cruz JS (2001) Effect of CSTX-1, a toxin from Cupiennius salei spider, on L-type calcium currents. XVI FESBE Annual Meeting , Abstract #20.030, pg 237, 2001

Malli H, Kuhn-Nentwig L, Imboden H, Moon MJ & Wyler T (2000) Immunocytochemical localization and secretion process of the toxin CSTX-1 in the venom gland of the wandering spider Cupiennius salei (Araneae: Ctenidae). Cell Tissue Res 299: 417-426

Haeberli S, Kuhn-Nentwig L, Schaller J & Nentwig W (2000) Characterisation of antibacterial activity of peptides isolated from the venom of the spider Cupiennius salei (Araneae: Ctenidae). Toxicon 38: 373-380

Kuhn-Nentwig L, Schaller J, Kämpfer U, Imboden H, Malli H, & Nentwig W (2000) A lysine rich C-terminal tail is directly involved in the toxicity of CSTX-1, a neurotoxic peptide from the venom of the spider Cupiennius salei. Arch Insect Biochem Physiol44: 101-111

Malli H, Kuhn-Nentwig L, Imboden H & Nentwig W (1999) Effects of size, motility and paralysation time of prey on the quantity of venom injected by the hunting spider Cupiennius salei. J Exp Biol202: 2083-2089

Nentwig W, Kuhn-Nentwig L, Schaller J & Kämpfer U (1998) Amino acid sequence of the toxic spider peptide CSTX-2 and its structural and physiological differences to CSTX-1. 12th World Congress on Animal, Plant and Microbial Toxins, Cuernavaca, Mexico, USA, September 21-26, 1997. Toxicon36: 1276

Malli H, Imboden H & Kuhn-Nentwig L (1998) Quantifying the venom dose of the spider Cupiennius salei using monoclonal antibodies. Toxicon36: 1959-1969

Kuhn-Nentwig L, Bücheler A, Studer A & Nentwig W (1998) Taurine and histamine: Low molecular compounds in prey hemolymph increase the killing power of spider venom. Naturwissenschaften85: 136-138

Kuhn-Nentwig L , Schaller J, Streb B & Kämpfer U (1998) CSTX-4, a novel bactericidal and insecticidal peptide in the venom of Cupiennius salei. 12th World Congress on Animal, Plant and Microbial Toxins, Cuernavaca, Mexico, USA, September 21-26, 1997. Toxicon36: 1276-1277

Kuhn Nentwig L , Nentwig W (1997) Venom of the hunting spider Cupiennius salei (Ctenidae). Toxicon 35: 813

Kuhn-Nentwig L & Malli H (1996) Purification and comparison of two toxic peptides (CSTX-1 and CSTX-2) in the venom of Cupiennius salei (Ctenidae). Revue Suisse de Zoologie, vol. hors série, 371-375

Boevé JL, Kuhn-Nentwig L, Keller S & Nentwig W (1995) Quantity and quality of venom released by a spider (Cupiennius salei, Ctenidae). Toxicon33: 1347-1357

Kuhn-Nentwig L, Schaller J & Nentwig W (1994) Purification of toxic peptides and the amino Acid sequence of CSTX-1 from the multicomponent venom of Cupiennius salei (Araneae: Ctenidae). Toxicon32: 287-302

Kuhn-Nentwig L & Nentwig W (1993) Zur Biochemie des Giftes von Cupienniussalei (Ctenidae). Bull Soc neuchatel Sci nat 116: 153-159

Kadenbach B, Büge U, Hüther F-J, Thiel C, Droste M, Schlerf A, Schneyder B, Kuhn-Nentwig L & Müller-Höcker J (1990) Regulation of activity and tissue-specific expression of cytochrome c oxidase genes under normal and pathological conditions. In: Bioenergetics; Molecular Biology, Biochemistry and Pathology (C. Kim and T. Ozawa, eds.) Plenum Publ. Corp., New York, 399-412

Kadenbach B, Kuhn-Nentwig L, Stroh A & Büge U (1987) Structure and regulatory function of cytochrome c oxidase isozymes. In: Biomembranes: Structure, Biogenesis and Transport. C. Rajamanickam, ed., Today + Tomorrow's Printers + Publishers, New Delhi, pp. 103-122

Kadenbach B, Kuhn-Nentwig L & Büge U (1987) Evolution of a regulatory enzyme: Cytochrome c oxidase (complex IV). Curr Top Bioenerg 15: 113-161

Kuhn-Nentwig L & Kadenbach B (1986) Isolation and characterization of human heart cytochrome c oxidase. J. Bioenerg. Biomembr. 18: 307-314

Kadenbach B, Stroh A, Ungibauer M, Kuhn-Nentwig L, Büge U & Jarausch J (1986) Isozymes of cytochrome c oxidase: characterization and isolation from different tissues. Methods Enzymol. 126: 32-45

Johnson MA, Kadenbach B, Kuhn-Nentwig L, Fulthorpe JF, Sherratt HSA & Turnbull DM (1985) Immunocytochemical studies of cytochrome c oxidase deficiencies in human skeletal muscle. Biochem Soc Trans 13: 729

Kuhn-Nentwig L & Kadenbach B (1985) Orientation of rat liver cytochrome c oxidase subunits investigated with subunit-specific antisera. Eur J Biochem 153: 101-104

Kuhn-Nentwig L & Kadenbach B (1985) Isolation and properties of cytochrome c oxidase from rat liver and quantification of immunological differences between isozymes from various rat tissues with subunit-specific antisera. Eur J Biochem 149 : 147-158

Kuhn-Nentwig L (1985) Immunologische Untersuchungen zur Struktur der Rattenleber-Cytochrom c Oxidase. Dissertation, Fachbereich Biologie, Philipps Universität Marburg, Germany.

Kadenbach B, Stroh A & Kuhn-Nentwig L (1984) Isozymes and polymorphism of mammalian cytochrome c oxidase. Third European Bioenergetics Conference, Short Reports 3: 59-60

Kuhn-Nentwig L, Jarausch J, Stroh A, Ungibauer M & Kadenbach B (1984) Aarrangement of subunits in the mammalian cytochrome c oxidase. Hoppe-Seyler’s Z. Physiol. Chem. 365 : 1022

Kuhn-Nentwig L & Kadenbach B (1984) Immunological identification of four different polypeptides in "subunit VII" of mammalian cytochrome c oxidase. FEBS Lett. 172: 189-192

Kuhn-Nentwig L & Kadenbach B (1983) Different immunological relationship between cytochrome c oxidase polypeptides from various tissues of the rat. Hoppe-Seyler’s Z Physiol Chem 364 : 1168

Kadenbach B, Ungibauer M, Jarausch J, Büge U & Kuhn-Nentwig L (1983) The complexity of respiratory complexes. Trends Biochem Sci 8: 398-400

Heubel F, Hedrich HJ & Kuhn-Nentwig L (1981) Cytochrome P 450: sources of variability in mice. Naunyn-Schmiedeberg’s Arch 316 : R6 (22)

Kuhn-Nentwig L (1981) Einfluss biologischer Faktoren auf die Aktivität des Cytochrom P 450 in der Leber nicht induzierter Mäuse. Diplomarbeit, Fachbereich Biologie, Philipps Universität Marburg, Germany.

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